Description
Accession
P26717
Source
DNA sequence encoding Human NKG2C&CD94 including a N-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human NKG2C&CD94 is a protein consisting of amino acid residues [Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)] ,The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKG2C&CD94 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human NKG2C&CD94 Protein Tris-Bis PAGE
- Human NKG2C&CD94 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human NKG2C&CD94 Protein SEC-HPLC
- The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2C&CD94 Protein SPR Data
- Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay (Biacore T200).
