Description
Accession
P26715
Source
DNA sequence encoding Human NKG2A&CD94 including a C-mFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human NKG2A&CD94 is a protein consisting of amino acid residues [Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)] ,The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKG2A&CD94 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human NKG2A&CD94 Protein Tris-Bis PAGE
- Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human NKG2A&CD94 Protein SEC-HPLC
- The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2A&CD94 Protein ELISA Data
- Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.
Human NKG2A&CD94 Protein Blocking Data
- Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 80.0ng/ml.
Human NKG2A&CD94 Protein SPR Data
- Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
