Description
Accession
Q92692
Source
DNA sequence encoding Human Nectin-2/CD112 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Nectin-2/CD112 is a protein consisting of amino acid residues [Gln32-Leu360] ,The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Nectin-2/CD112 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human Nectin-2/CD112 Protein Tris-Bis PAGE
- Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Nectin-2/CD112 Protein SEC-HPLC
- The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
Human Nectin-2/CD112 Protein ELISA Data
- Immobilized Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.80μg/ml determined by ELISA.
Human Nectin-2/CD112 Protein SPR Data
- Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).
Human Nectin-2/CD112 Protein SPR Data
- Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200).
