Description
Accession
Q15223
Source
DNA sequence encoding Human Nectin-1/PVRL1/CD111 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Nectin-1/PVRL1/CD111 is a protein consisting of amino acid residues [Gln31-Thr334] ,The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Nectin-1/PVRL1/CD111 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Q96NY8
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human Nectin-1/PVRL1/CD111 Protein Tris-Bis PAGE
- Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Nectin-1/PVRL1/CD111 Protein SEC-HPLC
- The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC.
Human Nectin-1/PVRL1/CD111 Protein SPR Data
- Human Nectin-4, hFc Tag captured on Protein A chip, can bind Human Nectin-1, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200).
Human Nectin-1/PVRL1/CD111 Protein SPR Data
- Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200).
