Description
Accession
Q13421
Source
DNA sequence encoding Human MSLN/Mesothelin including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human MSLN/Mesothelin is a protein consisting of amino acid residues [Glu296-Gly580] ,The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human MSLN/Mesothelin was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human MSLN/Mesothelin Protein Tris-Bis PAGE
- Human MSLN on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human MSLN/Mesothelin Protein ELISA Data
- Immobilized Human MSLN at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 29.2ng/ml determined by ELISA.
Human MSLN/Mesothelin Protein ELISA Data
- Immobilized Human CA125, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 2.8μg/ml determined by ELISA.
Human MSLN/Mesothelin Protein 37℃ Stability Test
- Immobilized Human MSLN Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-Human MSLN Antibody, hFc Tag with the EC50 of 0.1/ 0.1/ 0.1μg/ml determined by ELISA.
