Description
Accession
P22894
Source
DNA sequence encoding Human MMP-8 (pro form) including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human MMP-8 (pro form) is a protein consisting of amino acid residues [Phe21-Gly467] ,The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human MMP-8 (pro form) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Human MMP-8 Protein (pro form) Tris-Bis PAGE
- Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human MMP-8 Protein (pro form) SEC-HPLC
- The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Human MMP-8 Protein (pro form) ELISA Data
- Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5μg/ml determined by ELISA.
Human MMP-8 Protein (pro form) SPR Data
- Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 μM as determined in SPR assay (Biacore T200).
