Description
Accession
P07333
Source
DNA sequence encoding Human M-CSF R/CSF1R/CD115 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human M-CSF R/CSF1R/CD115 is a protein consisting of amino acid residues [Ile20-Glu512] ,The protein has a predicted MW of 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human M-CSF R/CSF1R/CD115 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Methods
Human M-CSF R/CSF1R/CD115 Protein Tris-Bis PAGE
- Human M-CSF R Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human M-CSF R/CSF1R/CD115 Protein SEC-HPLC
- The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.
Human M-CSF R/CSF1R/CD115 Protein ELISA Data
- Immobilized Human M-CSF R, His Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.
