Description
Accession
O75581
Source
DNA sequence encoding Human LRP-6 including a C-mFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LRP-6 is a protein consisting of amino acid residues [Ala20-Pro630] ,The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LRP-6 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O35468
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
P47876
Interactor
Interactor
P62988
Interactor
Interactor
Interactor
Q9ULT6
Methods
Human LRP-6 Protein Tris-Bis PAGE
- Human LRP-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human LRP-6 Protein SEC-HPLC
- The purity of Human LRP-6 is greater than 95% as determined by SEC-HPLC.
Human LRP-6 Protein ELISA Data
- Immobilized Human DKK1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.1μg/ml determined by ELISA.
