Description
Accession
AAH26309
Source
DNA sequence encoding Human LILRB4/CD85k/ILT3 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LILRB4/CD85k/ILT3 is a protein consisting of amino acid residues [Gln22-Arg256] ,The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LILRB4/CD85k/ILT3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human LILRB4/CD85k/ILT3 Protein Tris-Bis PAGE
- Human LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human LILRB4/CD85k/ILT3 Protein SEC-HPLC
- The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
Human LILRB4/CD85k/ILT3 Protein ELISA Data
- Immobilized Human LILRB4, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Human LILRB4/CD85k/ILT3 Protein SPR Data
- Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).
