Description
Accession
Q8NHL6
Source
DNA sequence encoding Human LILRB1/CD85j/ILT2 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LILRB1/CD85j/ILT2 is a protein consisting of amino acid residues [Gly24-His458] ,The protein has a predicted MW of 73.8 kDa. Due to glycosylation, the protein migrates to 78-105 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LILRB1/CD85j/ILT2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human LILRB1/CD85j/ILT2 Protein Tris-Bis PAGE
- Human LILRB1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human LILRB1/CD85j/ILT2 Protein SEC-HPLC
- The purity of Human LILRB1 is greater than 95% as determined by SEC-HPLC.
Human LILRB1/CD85j/ILT2 Protein ELISA Data
- Immobilized Human LILRB1, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-LILRB1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Human LILRB1/CD85j/ILT2 Protein SPR Data
- Human LILRB1, hFc Tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 1.49nM as determined in SPR assay (Biacore T200).
