Human LILRB1/CD85j/ILT2 Domain1&2 Protein Recombinant
Category: Receptors$216.00 – $721.00
Description
Accession
Q8NHL6
Source
DNA sequence encoding Human LILRB1/CD85j/ILT2 Domain1&2 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LILRB1/CD85j/ILT2 Domain1&2 is a protein consisting of amino acid residues [Gly24-Gly221] ,The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LILRB1/CD85j/ILT2 Domain1&2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human LILRB1/CD85j/ILT2 Domain1&2 Protein Tris-Bis PAGE
- Human LILRB1 Domain1&2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human LILRB1/CD85j/ILT2 Domain1&2 Protein SEC-HPLC
- The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.
Human LILRB1/CD85j/ILT2 Domain1&2 Protein ELISA Data
- Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Human LILRB1/CD85j/ILT2 Domain1&2 Protein Blocking Data
- Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.
Human LILRB1/CD85j/ILT2 Domain1&2 Protein SPR Data
- Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9μM as determined in SPR assay (Biacore T200).
