Description
Accession
P42702
Source
DNA sequence encoding Human LIF R/CD118 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LIF R/CD118 is a protein consisting of amino acid residues [Gln45-Ser833] ,The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LIF R/CD118 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Methods
Human LIF R/CD118 Protein Tris-Bis PAGE
- Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human LIF R/CD118 Protein SEC-HPLC
- The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
Human LIF R/CD118 Protein ELISA Data
- Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA.
Human LIF R/CD118 Protein SPR Data
- Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).
