Description
Accession
Q6GTX8
Source
DNA sequence encoding Human LAIR1/CD305 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human LAIR1/CD305 is a protein consisting of amino acid residues [Gln22-His163] ,The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LAIR1/CD305 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Human LAIR1/CD305 Protein Tris-Bis PAGE
- Human LAIR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human LAIR1/CD305 Protein SEC-HPLC
- The purity of Human LAIR1 is greater than 95% as determined by SEC-HPLC.
Human LAIR1/CD305 Protein ELISA Data
- Immobilized Human LAIR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
Human LAIR1/CD305 Protein SPR Data
- Human LAIR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.082 nM as determined in SPR assay (Biacore T200).
