Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
P06756
Source
DNA sequence encoding Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer is a protein consisting of amino acid residues [Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail] ,The protein has a predicted MW of 131.90 kDa (ITGAV)&101.79 kDa (ITGB3). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAV)&105-130 kDa (ITGB3) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P03231
Interactor
Interactor
P05106
Interactor
P18564
Methods
Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein Tris-Bis PAGE
- Human ITGAV&ITGB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein SEC-HPLC
- The purity of Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC.
Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein ELISA Data
- Immobilized Human ITGAV&ITGB3, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA.
