Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
P08648
Source
DNA sequence encoding Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer is a protein consisting of amino acid residues [Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail] ,The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P97333
Methods
Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein Tris-Bis PAGE
- Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein SEC-HPLC
- The purity of Human ITGA5&ITGB1 is greater than 95% as determined by SEC-HPLC.
Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein ELISA Data
- Immobilized Human ITGA5&ITGB1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
