Description
Accession
P08887
Source
DNA sequence encoding Human IL-6 R alpha/CD126 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-6 R alpha/CD126 is a protein consisting of amino acid residues [Leu20-Pro365] ,The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-6 R alpha/CD126 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Methods
Human IL-6 R alpha/CD126 Protein Tris-Bis PAGE
- Human IL-6 R alpha on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human IL-6 R alpha/CD126 Protein SEC-HPLC
- The purity of Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-6 R alpha/CD126 Protein ELISA Data
- Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.25μg/ml determined by ELISA.
Human IL-6 R alpha/CD126 Protein SPR Data
- Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
