Description
Accession
P05112
Source
DNA sequence encoding Human IL-4 including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-4 is a protein consisting of amino acid residues [His25-Ser153] ,The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Molecular function
Molecular function
Methods
Human IL-4 Protein Tris-Bis PAGE
- Human IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-4 Protein SEC-HPLC
- The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.
Human IL-4 Protein ELISA Data
- Immobilized Human IL-4, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
Human IL-4 Protein Cell Based Assay
- Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL
