Description
Accession
Q8NI17
Source
DNA sequence encoding Human IL-31 RA including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-31 RA is a protein consisting of amino acid residues [Ala20-Glu519] ,The protein has a predicted MW of 83.8 kDa. Due to glycosylation, the protein migrates to 110-135 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-31 RA was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human IL-31 RA Protein Tris-Bis PAGE
- Human IL-31 RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-31 RA Protein SEC-HPLC
- The purity of Human IL-31 RA is greater than 95% as determined by SEC-HPLC.
Human IL-31 RA Protein ELISA Data
- Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA.
Human IL-31 RA Protein
- Immobilized Human IL-31 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
Human IL-31 RA Protein SPR Data
- Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200).
