Description
Accession
P26951
Source
DNA sequence encoding Human IL-3 R alpha/CD123 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-3 R alpha/CD123 is a protein consisting of amino acid residues [Thr19-Arg305] ,The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-3 R alpha/CD123 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human IL-3 R alpha/CD123 Protein Tris-Bis PAGE
- Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-3 R alpha/CD123 Protein SEC-HPLC
- The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-3 R alpha/CD123 Protein ELISA Data
- Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Human IL-3 R alpha/CD123 Protein SPR Data
- Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 μM as determined in SPR assay (Biacore T200).
