Description
Accession
Q5VWK5
Source
DNA sequence encoding Human IL-23R including a C-mFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-23R is a protein consisting of amino acid residues [Gly24-Gly355] ,The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-23R was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Methods
Human IL-23R Protein Tris-Bis PAGE
- Human IL-23R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-23R Protein SEC-HPLC
- The purity of Human IL-23R is greater than 95% as determined by SEC-HPLC.
Human IL-23R Protein ELISA Data
- Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA.
Human IL-23R Protein SPR Data
- Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200).
