Human IL-23 alpha&IL-12 beta Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
Q9NPF7
Source
DNA sequence encoding Human IL-23 alpha&IL-12 beta including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-23 alpha&IL-12 beta is a protein consisting of amino acid residues [Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)] ,The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-23 alpha&IL-12 beta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Biological Process
Molecular function
Methods
Human IL-23 alpha&IL-12 beta Protein Tris-Bis PAGE
- Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-23 alpha&IL-12 beta Protein ELISA Data
- Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Human IL-23 alpha&IL-12 beta Protein SPR Data
- Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200).
