Description
Accession
NP_000869
Source
DNA sequence encoding Human IL-2 R beta/CD122 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-2 R beta/CD122 is a protein consisting of amino acid residues [Ala27-Asp239] ,The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-2 R beta/CD122 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human IL-2 R beta/CD122 Protein Tris-Bis PAGE
- Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-2 R beta/CD122 Protein SEC-HPLC
- The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
Human IL-2 R beta/CD122 Protein SPR Data
- Human IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200).
Human IL-2 R beta/CD122 Protein
- Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200).
