Description
Accession
O95998
Source
DNA sequence encoding Human IL-18BP including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-18BP is a protein consisting of amino acid residues [Thr31-Gly194] ,The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-18BP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human IL-18BP Protein Tris-Bis PAGE
- Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-18BP Protein SEC-HPLC
- The purity of Human IL-18BP is greater than 95% as determined by SEC-HPLC.
Human IL-18BP Protein ELISA Data
- Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA.
Human IL-18BP Protein SPR Data
- Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay (Biacore T200).
