Description
Accession
Q9NRM6
Source
DNA sequence encoding Human IL-17RB including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-17RB is a protein consisting of amino acid residues [Arg18-Per292] ,The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-17RB was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human IL-17RB Protein Tris-Bis PAGE
- Human IL-17RB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-17RB Protein SEC-HPLC
- The purity of Human IL-17RB is greater than 95% as determined by SEC-HPLC.
Human IL-17RB Protein ELISA Data
- Immobilized Human IL-17RB, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human IL-17RB Protein
- Immobilized Human IL-17RB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
Human IL-17RB Protein SPR Data
- Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81 nM as determined in SPR assay (Biacore T200).
