Description
Accession
Q13261
Source
DNA sequence encoding Human IL-15RA&IL-15 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-15RA&IL-15 is a protein consisting of amino acid residues [Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)] ,The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-15RA&IL-15 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human IL-15RA&IL-15 Protein Tris-Bis PAGE
- Human IL-15RA&IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-15RA&IL-15 Protein SEC-HPLC
- The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.
Human IL-15RA&IL-15 Protein ELISA Data
- Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
Human IL-15RA&IL-15 Protein SPR Data
- Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
