Description
Accession
P78552
Source
DNA sequence encoding Human IL-13Ra1 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-13Ra1 is a protein consisting of amino acid residues [Ala27-Thr343] ,The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-13Ra1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human IL-13Ra1 Protein Tris-Bis PAGE
- Human IL-13Ra1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human IL-13Ra1 Protein SEC-HPLC
- The purity of Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
Human IL-13Ra1 Protein ELISA Data
- Immobilized Human IL-13 at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA.
Human IL-13Ra1 Protein SPR Data
- Human IL-13Ra1, hFc Tag captured on Protein A chip, can bind Human IL-13, His Tag with an affinity constant of 53.1nM as determined in a SPR assay (Biacore T200).
