Description
Accession
AAK53823
Source
DNA sequence encoding Human IL-13 including a N-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-13 is a protein consisting of amino acid residues [Gly21-Asn132] ,The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-13 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human IL-13 Protein Tris-Bis PAGE
- Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-13 Protein SEC-HPLC
- The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC.
Human IL-13 Protein ELISA Data
- Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 2.8μg/ml determined by ELISA.
Human IL-13 Protein SPR Data
- Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 μM as determined in SPR assay (Biacore T200).
