Description
Accession
P29460
Source
DNA sequence encoding Human IL-12B/p40/NKSF2 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IL-12B/p40/NKSF2 is a protein consisting of amino acid residues [Ile23-Ser328] ,The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-12B/p40/NKSF2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Methods
Human IL-12B/p40/NKSF2 Protein Tris-Bis PAGE
- Human IL-12B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IL-12B/p40/NKSF2 Protein ELISA Data
- Immobilized Human IL-12B, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Human IL-12B/p40/NKSF2 Protein SPR Data
- Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
