Description
Accession
P01861
Source
DNA sequence encoding Human IgG4 Fc including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IgG4 Fc is a protein consisting of amino acid residues [Glu99-Gly326] ,The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IgG4 Fc was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human IgG4 Fc Protein Tris-Bis PAGE
- Human IgG4 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IgG4 Fc Protein SEC-HPLC
- The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.
Human IgG4 Fc Protein SPR Data
- Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 μM as determined in SPR assay (Biacore T200).
