Description
Accession
P01857
Source
DNA sequence encoding Human IgG1 Fc including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IgG1 Fc is a protein consisting of amino acid residues [Asp103-Lys330] ,The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IgG1 Fc was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P04488
Biological Process
Methods
Human IgG1 Fc Protein Tris-Bis PAGE
- Human IgG1 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IgG1 Fc Protein SEC-HPLC
- The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.
Human IgG1 Fc Protein SPR Data
- Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.40 μM as determined in SPR assay (Biacore T200).
