Description
Accession
Q16270
Source
DNA sequence encoding Human IGFBP-7 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IGFBP-7 is a protein consisting of amino acid residues [Ser27-Leu282] ,The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IGFBP-7 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human IGFBP-7 Protein Tris-Bis PAGE
- Human IGFBP-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IGFBP-7 Protein ELISA Data
- Immobilized Human IGFBP-7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.83μg/ml determined by ELISA.
