Description
Accession
P01579
Source
DNA sequence encoding Human IFN gamma/IFNG including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human IFN gamma/IFNG is a protein consisting of amino acid residues [Gln24-Gly161] ,The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IFN gamma/IFNG was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q66793
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human IFN gamma/IFNG Protein Tris-Bis PAGE
- Human IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human IFN gamma/IFNG Protein SEC-HPLC
- The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC.
Human IFN gamma/IFNG Protein ELISA Data
- Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
