Description
Accession
Q9Y6W8
Source
DNA sequence encoding Human ICOS/CD278 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human ICOS/CD278 is a protein consisting of amino acid residues [Glu21-Phe141] ,The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human ICOS/CD278 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human ICOS/CD278 Protein Tris-Bis PAGE
- Human ICOS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human ICOS/CD278 Protein SEC-HPLC
- The purity of Human ICOS is greater than 95% as determined by SEC-HPLC.
Human ICOS/CD278 Protein ELISA Data
- Immobilized Human B7-H2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.
Human ICOS/CD278 Protein SPR Data
- Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
