Human ICOS/CD193 (C136S, C137S) Protein Recombinant
Category: Receptors$145.00 – $486.00
Description
Accession
Q9Y6W8
Source
DNA sequence encoding Human ICOS/CD278 (C136S, C137S) including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human ICOS/CD278 (C136S, C137S) is a protein consisting of amino acid residues [Glu21-Phe141(C136S,C137S)] ,The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human ICOS/CD278 (C136S, C137S) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human ICOS/CD278 (C136S, C137S) Protein Tris-Bis PAGE
- Human ICOS (C136S,C137S) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human ICOS/CD278 (C136S, C137S) Protein ELISA Data
- Immobilized Human ICOS (C136S,C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Human ICOS/CD278 (C136S, C137S) Protein SPR Data
- Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
