Description
Accession
Q92956
Source
DNA sequence encoding Human HVEM/TNFRSF14 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human HVEM/TNFRSF14 is a protein consisting of amino acid residues [Leu39-Val202] ,The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human HVEM/TNFRSF14 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q12933
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human HVEM/TNFRSF14 Protein Tris-Bis PAGE
- Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human HVEM/TNFRSF14 Protein SEC-HPLC
- The purity of Human HVEM is greater than 95% as determined by SEC-HPLC.
Human HVEM/TNFRSF14 Protein ELISA Data
- Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA.
Human HVEM/TNFRSF14 Protein
- Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA.
Human HVEM/TNFRSF14 Protein
- Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
