Description
Accession
Q15303
Source
DNA sequence encoding Human Her4/ErbB4 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Her4/ErbB4 is a protein consisting of amino acid residues [Gln26-Pro651] ,The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Her4/ErbB4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
P62988
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human Her4/ErbB4 Protein Tris-Bis PAGE
- Human Her4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Her4/ErbB4 Protein SEC-HPLC
- The purity of Human Her4 is greater than 95% as determined by SEC-HPLC.
Human Her4/ErbB4 Protein ELISA Data
- Immobilized Human Her4 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 34.7ng/ml determined by ELISA.
