Description
Accession
Q9HCN6
Source
DNA sequence encoding Human GPVI including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GPVI is a protein consisting of amino acid residues [Gln21-Lys267] ,The protein has a predicted MW of 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GPVI was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P07947
Interactor
P08631
Interactor
Q06187
Interactor
P06241
Interactor
Interactor
P46109
Interactor
Q15811
Methods
Human GPVI Protein Tris-Bis PAGE
- Human GPVI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GPVI Protein SEC-HPLC
- The purity of Human GPVI is greater than 95% as determined by SEC-HPLC.
Human GPVI Protein ELISA Data
- Immobilized Human GPVI, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.
