Description
Accession
P15509
Source
DNA sequence encoding Human GM-CSF R alpha including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GM-CSF R alpha is a protein consisting of amino acid residues [Glu23-Gly320] ,The protein has a predicted MW of 63.5 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GM-CSF R alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human GM-CSF R alpha Protein Tris-Bis PAGE
- Human GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GM-CSF R alpha Protein SEC-HPLC
- The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Human GM-CSF R alpha Protein ELISA Data
- Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
Human GM-CSF R alpha Protein
- Immobilized Human GM-CSF R alpha, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 0.18μg/ml determined by ELISA.
