Description
Accession
P04141
Source
DNA sequence encoding Human GM-CSF including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GM-CSF is a protein consisting of amino acid residues [Ala18-Glu144] ,The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GM-CSF was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Molecular function
Methods
Human GM-CSF Protein Tris-Bis PAGE
- Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GM-CSF Protein SEC-HPLC
- The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
Human GM-CSF Protein ELISA Data
- Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
Human GM-CSF Protein SPR Data
- Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Human GM-CSF Protein Cell Based Assay
- The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/mL.
