Description
Accession
Q9Y5U5
Source
DNA sequence encoding Human GITR/TNFRSF18 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GITR/TNFRSF18 is a protein consisting of amino acid residues [Gln26-Glu161] ,The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GITR/TNFRSF18 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Methods
Human GITR/TNFRSF18 Protein Tris-Bis PAGE
- Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%
Human GITR/TNFRSF18 Protein SEC-HPLC
- The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Human GITR/TNFRSF18 Protein ELISA Data
- Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA.
Human GITR/TNFRSF18 Protein ELISA Data
- Immobilized Human GITR, His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA.
Human GITR/TNFRSF18 Protein SPR Data
- Human GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay (Biacore T200).
