Human GITR Ligand/TNFSF18 Trimer Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
Q9UNG2
Source
DNA sequence encoding Human GITR Ligand/TNFSF18 Trimer including a N-monomeric hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GITR Ligand/TNFSF18 Trimer is a protein consisting of amino acid residues [Gln50-Ser177] ,The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GITR Ligand/TNFSF18 Trimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Biological Process
Molecular function
Methods
Human GITR Ligand/TNFSF18 Trimer Protein Tris-Bis PAGE
- Human GITR Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GITR Ligand/TNFSF18 Trimer Protein SEC-HPLC
- The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Human GITR Ligand/TNFSF18 Trimer Protein SPR Data
- Human GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand (Trimer) , hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay (Biacore T200).
