Description
Accession
Q6UXV0
Source
DNA sequence encoding Human GFRAL/GFR alpha-like including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GFRAL/GFR alpha-like is a protein consisting of amino acid residues [Ser19-Glu351] ,The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GFRAL/GFR alpha-like was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human GFRAL/GFR alpha-like Protein Tris-Bis PAGE
- Human GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GFRAL/GFR alpha-like Protein SEC-HPLC
- The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.
Human GFRAL/GFR alpha-like Protein ELISA Data
- Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15ng/ml determined by ELISA.
Human GFRAL/GFR alpha-like Protein SPR Data
- Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
