Description
Accession
P39905
Source
DNA sequence encoding Human GDNF including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GDNF is a protein consisting of amino acid residues [Ser78-Ile211] ,The protein has a predicted MW of 15.06 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GDNF was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q8N5Y2
Interactor
Molecular function
Methods
Human GDNF Protein Tris-Bis PAGE
- Human GDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human GDNF Protein SEC-HPLC
- The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.
Human GDNF Protein ELISA Data
- Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA.
