Description
Accession
Q99988
Source
DNA sequence encoding Human GDF15 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human GDF15 is a protein consisting of amino acid residues [Ala197-Ile308] ,The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GDF15 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Methods
Human GDF15 Protein Tris-Bis PAGE
- Human GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Human GDF15 Protein ELISA Data
- Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA.
Human GDF15 Protein Blocking Data
- Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
Human GDF15 Protein SPR Data
- Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
