Description
Accession
P49771
Source
DNA sequence encoding Human FLT3 Ligand including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FLT3 Ligand is a protein consisting of amino acid residues [Thr27-Pro185] ,The protein has a predicted MW of 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FLT3 Ligand was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human FLT3 Ligand Protein Tris-Bis PAGE
- Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human FLT3 Ligand Protein ELISA Data
- Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Human FLT3 Ligand Protein SPR Data
- Human FLT3 Ligand, hFc Tag immobilized on CM5 Chip can bind Mouse FLT3, hFc Tag with an affinity constant of 35.07 nM as determined in SPR assay (Biacore T200).
