Description
Accession
P21802
Source
DNA sequence encoding Human FGFR2 beta (IIIb) including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FGFR2 beta (IIIb) is a protein consisting of amino acid residues [Arg152-Glu378] ,The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FGFR2 beta (IIIb) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
P11487
Interactor
Interactor
P50281
Molecular function
Molecular function
Molecular function
Methods
Human FGFR2 beta (IIIb) Protein Tris-Bis PAGE
- Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human FGFR2 beta (IIIb) Protein SEC-HPLC
- The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
Human FGFR2 beta (IIIb) Protein ELISA Data
- Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 lllb Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA.
Human FGFR2 beta (IIIb) Protein Blocking Data
- Serial dilutions of Anti-FGFR2 lllb Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.
Human FGFR2 beta (IIIb) Protein SPR Data
- Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).
