Description
Accession
P21802
Source
DNA sequence encoding Human FGFR2 alpha (IIIb) including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FGFR2 alpha (IIIb) is a protein consisting of amino acid residues [Arg22-Glu378] ,The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FGFR2 alpha (IIIb) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
P11487
Interactor
Interactor
P50281
Molecular function
Molecular function
Molecular function
Methods
Human FGFR2 alpha (IIIb) Protein Tris-Bis PAGE
- Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human FGFR2 alpha (IIIb) Protein SEC-HPLC
- The purity of Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Human FGFR2 alpha (IIIb) Protein ELISA Data
- Immobilized Human FGFR2 alpha (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.
Human FGFR2 alpha (IIIb) Protein Blocking Data
- Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins.
