Description
Accession
P11362-7
Source
DNA sequence encoding Human FGFR1 alpha (IIIc) including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FGFR1 alpha (IIIc) is a protein consisting of amino acid residues [Arg22-Glu374] ,The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FGFR1 alpha (IIIc) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human FGFR1 alpha (IIIc) Protein Tris-Bis PAGE
- Human FGFR1 alpha (IIIc) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human FGFR1 alpha (IIIc) Protein SEC-HPLC
- The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.
Human FGFR1 alpha (IIIc) Protein ELISA Data
- Immobilized Human FGFR1 alpha (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA.
