Description
Accession
P09038
Source
DNA sequence encoding Human FGF basic including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FGF basic is a protein consisting of amino acid residues [Pro143-Ser288] ,The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FGF basic was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O35082
Interactor
O43353
Interactor
Interactor
Interactor
Q802A9
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human FGF basic Protein Tris-Bis PAGE
- Human FGF basic on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human FGF basic Protein ELISA Data
- Immobilized Human FGF basic at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 5.6ng/ml determined by ELISA.
Human FGF basic Protein ELISA Data
- Immobilized Human FGF basic at 5μg/ml (100μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc) , His Tag with the EC50 of 0.29μg/ml determined by ELISA.
