Description
Accession
P55899
Source
DNA sequence encoding Human FcRn including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human FcRn is a protein consisting of amino acid residues [Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)] ,The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FcRn was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Molecular function
Methods
Human FcRn Protein Tris-Bis PAGE
- Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human FcRn Protein SEC-HPLC
- The purity of Human FcRn is greater than 95% as determined by SEC-HPLC.
Human FcRn Protein ELISA Data
- Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
Human FcRn Protein SPR Data
- Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.40 μM as determined in SPR assay (Biacore T200).
